Part:BBa_K3875000:Design

gadB-E89Q+△452-466

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 700
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The wild type gadB gene was amplified from E. coli BW.25113 by using primers. Then introduce E89Q mutant andΔ452-466 mutation was introduce to wild type gadB gene using primers, resulting in PCS27-gadB-E89Q+z452-466-lac-gadA-T1.

Source

https://asap.genetics.wisc.edu/asap/feature_info.php?FeatureID=ABE-0004976 ABE-0004976 (gadB) - Escherichia coli K-12 MG1655 - Feature Annotation